Celebration of Scholarship and Creative Activity 2021
Mild hyperthermia plus ABT-263 induces apoptotic-like characters in HeLa cells that are arrested in metaphase. However, they fail to induce apoptosis in interphase HeLa cells. We hypothesized that it could be due to three of the following reasons. First, we believe that it could be due to the fact that MCl-1 (myeloid cell leukemia- 1) naturally goes down during mitosis. During mitosis, MCl-1 levels drop resulting in intracellular activities that induce apoptosis. Secondly, an anti-apoptotic protein called B-cell lymphoma-2 (Bcl-2) decreases in amount when Abt-263 is added. Abt-263 inhibits Bcl-2 protein production, thereby promoting apoptosis. Thirdly, we believe that Bim ―a pro-apoptotic protein that is attached to microtubules released during mitosis has an effect in inducing apoptosis. So, it could be that Bim not being released in interphase cells prevents apoptosis. So, with all these three hypotheses, we believe that activating BIM and inhibiting Mcl-1 in interphase cells could induce apoptosis. The idea being that since BIM activation during mitosis induced apoptosis, forcefully activating it in interphase cells could induce apoptosis. Likewise, imitating the decrease of MCl-1 that is seen in mitotic cells in Metaphase cells could induce apoptosis.